Catalase crystals, 3.05mm grid

51-1625-1630

Negatively stained catalase crystals show lattice plane spacings of approximately 8.75nm and 6.85nm very clearly (using TEM and STEM). They are valuable for high magnification calibration.

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DESCRIPTION

Negatively stained catalase crystals show lattice plane spacings of approximately 8.75nm and 6.85nm very clearly (using TEM and STEM). (Figures determined by Wrigley. J. Ultrastructure Res. 24, 454. 1968).

They are valuable for high magnification calibration.

For higher magnifications, one of the crystal lattice plane specimens can be used. Diffraction pattern ring diameters can be simply related to the lattice plane spacings in the crystal used as the specimen. For a lattice spacing d, in a microscope of effective camera length L and operating at an accelerating voltage corresponding to a wavelength l, the diffraction ring diameter is r where lL = dr.

It is not easy to measure the actual value of L or know the precise value of accelerating voltage. However, without knowing the exact values of l and L, one can determine an unknown lattice spacing by measurement of r, if the instrument has first been calibrated with a known substance. This can best be done by using a polycrystalline material of known lattice spacings, so that continuous diffraction rings are available for measurement. The ring diameters must all be measured in the same plane relative to the microscope (to avoid any errors due to ellipticity of the pattern).

Furthermore, the calibrating ring diameter chosen should be similar to that required to be calibrated since there may be distortion of the relative ring diameters due to residual barrel or pincushion distortion in the projector lens system.

The main lattice spacings of catalase crystals (8.75nm and 6.85nm) may be used to calibrate the camera length of a transmission microscope operating in high dispersion diffraction; that is, with the objective switched off and with illumination focused by condenser 2 on to the object plane of the intermediate lens.

 

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Our eStore allows customers to pay for their order by prepaid methods like debit & credit card, paypal or prepaid invoice via a bank transfer and through postpaid net 30 days invoice. If you wish to pay by card or paypal, you don’t need to communicate with us first and you can also check out as guest. If you wish to pay by prepaid bank transfer, you will have to register a MyOI account first, then request to ecommerceteam@estore.oxinst.com to activate prepaid bank transfer (Proforma invoice) on your MyOI account.

If you wish to pay by net 30 days invoice, you will have to request this when you register a MyOI account. Please be aware that only customers with previous business with Oxford Instruments will be authorised to pay by net 30 day invoice. You can explain your previous business with Oxford Instruments when you register your MyOI account. If your institution or company doesn’t have any previous business relationship with Oxford Instruments, you will be asked to place at least 3 prepaid orders through the eStore before we can consider your request.

If your institution or company has a special arrangement with Oxford Instruments, please get in touch with ecommerceteam@estore.oxinst.com providing information about your contact person in Oxford Instruments

Our eStore is managed by our ecommerce partner ModusLink. This means that on the e-invoice, you will see both the ModusLink and Oxford Instruments logos. If you wish to pay by net 30 day invoice, you will have to send us a PO with ModusLink as supplier, because the invoice will have to be paid into ModusLink’s bank account, so we need to make sure ModusLink is a registered vendor in your system and payment won’t be delayed.

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