Araldite CY212 Resin 500g
Araldite® - this epoxy resin is also known as Araldite M. This kit has proved to be extremely reliable and versatile for a range of tissues. It is easy to section. The sections are stable in the electron beam.
The kit consists of:
Araldite CY 212 (or Araldite M) was the first epoxy resin embedding medium developed for electron
microscopy (see Glauert, A.M. and Glauert, R.H., 1958, J. biopyhs. biochem. Cytol 4, 191-194).
The suggested standard embedding medium is as follows:
Araldite CY 212 (M) 20ml (23.0g)
DDSA 22ml (22.0g)
BDMA (C.3%) 1.1ml (1.2g)
Softer blocks may be obtained by adding a small amount of the plasticiser, dibutyl phthalate (e.g.
1.0ml to the standard embedding medium). For harder blocks, replace 1.0ml of the DDSA with 0.5ml of the hardener, methyl nadic anhydride (MNA).Complete mixing of the components of epoxy resin embedding media is very important and is facilitated if the Araldite resin and the DDSA hardener, and a graduated cylinder and a conical flask,are warmed to 60°C before mixing.
Measure the amounts (by volume) of the warm resin and hardener (from their separate containers)
into the warm graduated cylinder and pour them immediately into the warm conical flask. Shake the
mixture gently by hand with rotation. Mixing will be complete within a few minutes if the components
and the mixing vessel have been pre-warmed. Then add the BDMA (1.1ml for every 20ml of Araldite
and 22ml of DDSA) and continue shaking by hand for a further minute or two.
This embedding medium hardens overnight at 60°C, but longer times of 24 to 48 hours may slightly
improve the sectioning properties of the final block. The curing temperature should not be higher
than 60°C.
For more information, download the MSDS sheet here
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