Melinex® film is a convenient substrate for growing cell cultures and for later incorporation into blocks. Dimensions: 210 x 297mm, 175µm thick. Pack of 5 sheets.
Melinex is a polyester film. Thickness 175μm. The procedure is as follows:
1. Cut out coverslips of the required size from the Melinex sheet (e.g. 20mm x 20mm). It is important
to do this before growing the cells.
2. Wash with ethanol, by wiping with tissue soaked in ethanol.
3. Sterilise in ethanol (70%), 2 or more changes, or keep in 70% ethanol.
4. Air dry and keep sterile.
5. Incubate in serum-containing medium in a Petri dish overnight at 37°C (this removes any toxic
substances).
6. Remove medium, and wash in fresh medium.
7. Add cells and medium and incubate in the usual way.
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